DS-SPcasN-
(Plasmid #48657)

• Purpose: Bacterial nuclease-null SP Cas9 expression
• Depositing Lab: George Church
• Publication: Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.

Backbone

• Vector backbone: cloDF13-aadA
• Total vector size (bp): 6806
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cas9, nuclease-null
• Species: S. pyogenes
• Insert Size (bp): 4100
• Mutation: D to A, DH to AA, N to A
• Promoter: proC

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: rrnB-T2-term
• 3′ sequencing primer: StrepPyoCas9-5UTR-F

Gene/Insert 2

• Gene/Insert name: tracrRNA precursor
• Species: S. pyogenes
• Insert Size (bp): 100
• Promoter: tracdrRNA promoter

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: StrepPyoCas9-5UTR-F

Resource Information

• Supplemental Documents:
  • DS-SPcasN- genbank
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry