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PurposeBacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonecloDF13-aadA
- Total vector size (bp) 6028
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCas9
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SpeciesS. thermophilus #1
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Insert Size (bp)3360
- Promoter proC
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer rrnB-T2-term
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameST1 tracrRNA
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SpeciesS. thermophilus #1
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Insert Size (bp)200
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DS-ST1cas was a gift from George Church (Addgene plasmid # 48647 ; http://n2t.net/addgene:48647 ; RRID:Addgene_48647) -
For your References section:
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. 10.1038/nmeth.2681 PubMed 24076762
