pCS2TAL3-DDD
(Plasmid
#48637)
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Purpose(Empty Backbone) Expression of TALEN proteins with FokI-DDD domain
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48637 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2
- Backbone size (bp) 5768
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Modifications to backboneCloned in TALEN expression sequence
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Vector typeTALEN
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer Tal Seq FokI Rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2TAL3-DDD was a gift from David Grunwald (Addgene plasmid # 48637 ; http://n2t.net/addgene:48637 ; RRID:Addgene_48637) -
For your References section:
Targeting human microRNA genes using engineered Tal-effector nucleases (TALENs). Hu R, Wallace J, Dahlem TJ, Grunwald DJ, O'Connell RM. PLoS One. 2013 May 7;8(5):e63074. doi: 10.1371/journal.pone.0063074. Print 2013. 10.1371/journal.pone.0063074 PubMed 23667577
Map uploaded by the depositor.
