ERroGFP_iE_C204Q_pCDNA3
(Plasmid #48629)

• Purpose: Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)
• Depositing Lab: David Ron
• Publication: Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49. doi: 10.1083/jcb.201211155.

Backbone

• Vector backbone: pcDNA3 derivative
• Backbone size w/o insert (bp): 5370
• Total vector size (bp): 6194
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERroGFP
• Species: jellyfish
• Insert Size (bp): 824
• Mutation: insertion E147B, C204Q
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: James Remington U.Oregon USA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry