pcDNA3.BRWD3-V5H
(Plasmid #48624)

• Purpose: Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cells
• Depositing Lab: Aziz Sancar
• Publication: Ozturk et al Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):4980-5. doi: 10.1073/pnas.1303234110. Epub 2013 Mar 11.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 12236
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BRWD3
• Alt name: Ramshackle
• Species: D. melanogaster (fly)
• Insert Size (bp): 6808
• Mutation: no mutations, stop was removed to fuse V5His at C-ter.
• Entrez Gene: BRWD3 (a.k.a. Dmel_CG31132, CG18670, CG31132, CG6400, Dmel\CG31132, anon-WO0118547.520, anon-WO0118547.620, dBRWD3, l(3)05842, l(3)s5349, ram)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 and His tags (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: T7 for
• 3′ sequencing primer: BGH Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry