pDONR P4-P1R-V5-6xHis
(Plasmid #48346)

• Purpose: Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
• Depositing Labs: Anna Planas, Tomas Santalucia
• Publication: Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.

Backbone

• Vector backbone: pDONRP4-P1R
• Backbone manufacturer: Life Technologies/Invitrogen
• Backbone size w/o insert (bp): 2645
• Total vector size (bp): 2745
• Vector type: Gateway entry clone

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: V5 and 6xHis epitope tags cassette
• Species: Synthetic
• Insert Size (bp): 100
• Mutation: Contains a Kozak sequence and initiation codon upstream of V5 tag. Does not contain a stop codon.
• Promoter: none

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 forward (-20)
• 3′ sequencing primer: M13 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Life Technologies

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry