pDONR P2R-P3-mKate2
(Plasmid #48345)

• Purpose: Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
• Depositing Labs: Anna Planas, Tomas Santalucia
• Publication: Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18.

Backbone

• Vector backbone: pDONRP2R-P3
• Backbone manufacturer: Life Technologies/Invitrogen
• Backbone size w/o insert (bp): 2639
• Total vector size (bp): 3431
• Vector type: Gateway entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mKate2
• Species: Synthetic; Entacmaea quadricolor
• Insert Size (bp): 792
• Mutation: Contains a stop codon at the 3' end of the coding sequence.
• GenBank ID:
• Promoter: none

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 forward (-20)
• 3′ sequencing primer: M13 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Evrogen
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry