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Addgene

CAG-Flex-RG
(Plasmid #48333)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 48333 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7663
  • Modifications to backbone
    lox2272(c)-loxP(c)-SalI-AscI-lox2272-loxP cassette was inserted between CAG promoter and WPRE-hGHpA.
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rabies Glycoprotein
  • Insert Size (bp)
    1575
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site Asc1 (not destroyed)
  • 5′ sequencing primer TriEXUP.5
  • 3′ sequencing primer pWPTrev
    • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The plasmid was modified after a construct received from the Uchida lab at Harvard.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-Flex-RG was a gift from Liqun Luo (Addgene plasmid # 48333 ; http://n2t.net/addgene:48333 ; RRID:Addgene_48333)

  • For your References section:

    Dissecting local circuits: parvalbumin interneurons underlie broad feedback control of olfactory bulb output. Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A. Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027. Epub 2013 Nov 14. 10.1016/j.neuron.2013.08.027 PubMed 24239125
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