CAG-Flex-TC66T
(Plasmid #48331)

• Purpose: Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA
• Depositing Lab: Liqun Luo
• Publication: Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027. Epub 2013 Nov 14.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 7234
• Modifications to backbone: lox2272(c)-loxP(c)-SalI-AscI-lox2272-loxP cassette was inserted between CAG promoter and WPRE-hGpA.
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TVA receptor mCherry fusion with the 66T mutation
• Insert Size (bp): 1149
• Mutation: 66T
• Promoter: CAG
• Tag / Fusion Protein:
  • mCherry fusion to TVA receptor

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: Asc1 (not destroyed)
• 5′ sequencing primer: TriEXUP.5
• 3′ sequencing primer: pWPTRev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Modified after a construct received from the Uchida lab at Harvard
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry