pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)
(Plasmid #48294)

• Purpose: (Empty Backbone)
• Depositing Lab: Scott Gradia
• Publication: Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. Epub 2017 May 15.

Backbone

• Vector backbone: pFastBac
• Backbone size (bp): 5392
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is a LIC-adapted pFastBac vector.

Add the following tags to your PCR primers:
vBac Forward Tag TACTTCCAATCCAATCG
LicV1 Reverse Tag TTATCCACTTCCAATGTTATTA

Linearize this plasmid with SspI and gel purify the product, then T4-treat with dGTP. For the PCR product, T4-treat with dCTP.

Series 11 vectors have BioBrick restriction sites to facilitate subcloning reactions to make polycistronic expression vectors. NotI, PacI, AsiSI, and SbfI are the restriction enzyme sites that flank your open reading frame. For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/