pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C)
(Plasmid #48286)

• Purpose: (Empty Backbone)
• Depositing Lab: Scott Gradia
• Publication: Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with Biobrick Polycistronic Restriction Sites (unpublished)

Backbone

• Vector backbone: pET
• Backbone size (bp): 5929
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • His6 (N terminal on backbone)
  • MBP (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is an empty vector to be used with a LIC cloning protocol.

It has a TEV cleavable His6-MBP fusion tag on the N-terminus.

To clone into this vector, add LIC fusion tags to the 5' end of your PCR primers.
Forward - 5'TACTTCCAATCCAATGCA3'
Reverse - 5'TTATCCACTTCCAATGTTATTA3'

Linearize the plasmid with SspI and gel purify.

When digesting the DNA with T4 polymerase for LIC, use dCTP for insert and dGTP for vector. Series 9 vectors have BioBrick restriction sites to facilitate subcloning reactions to make polycistronic expression vectors. NotI, PacI, AsiSI, and SbfI are the restriction enzyme sites that flank your open reading frame. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/