pJH124
(Plasmid #48259)

• Purpose: Template plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.
• Depositing Lab: Ronald Davis
• Publication: Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.1002/yea.3006. Epub 2014 Mar 20.

Backbone

• Vector backbone: pBluescript II SK (-)
• Backbone manufacturer: Agilent Technologies
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 4605
• Vector type: Routine cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: URA3 3' fragment
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 669
• Mutation: URA3 ORF from +216 to 80 bp downstream of the stop codon

Gene/Insert 2

• Gene/Insert name: URA3 5' fragment
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 737
• Mutation: -242 upstream to URA3 ORF +495

Gene/Insert 3

• Gene/Insert name: Ag TEF1 promoter
• Species: Ashbya gossypii
• Insert Size (bp): 284
• Promoter: Ag TEF1 promoter

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: URA3 confC (custom primer)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pJH124 was created by cloning an XbaI/XhoI Ag TEF1 promoter fragment into the same sites of vector IpO. The cloned insert was confirmed by DNA sequencing.