PNXGW
(Plasmid
#48256)
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Purpose(Empty Backbone) Split-GFP assay in plants
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48256 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRT100/pPZP312
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Vector typeBinary Gateway vector
- Promoter 35S
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Selectable markersBasta
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Tags
/ Fusion Proteins
- 6xHIS (N terminal on backbone)
- YFP aa1-172 (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer 35S-promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PNXGW was a gift from Wolf Frommer (Addgene plasmid # 48256 ; http://n2t.net/addgene:48256 ; RRID:Addgene_48256) -
For your References section:
Xanthomonas T3S Effector XopN Suppresses PAMP-Triggered Immunity and Interacts with a Tomato Atypical Receptor-Like Kinase and TFT1. Kim JG, Li X, Roden JA, Taylor KW, Aakre CD, Su B, Lalonde S, Kirik A, Chen Y, Baranage G, McLane H, Martin GB, Mudgett MB. Plant Cell. 2009 Apr;21(4):1305-23. doi: 10.1105/tpc.108.063123. Epub 2009 Apr 14. 10.1105/tpc.108.063123 PubMed 19366901
Map uploaded by the depositor.