LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA
(Plasmid
#48253)
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PurposeLITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48253 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV_hSyn_WPRE_bGHpA
- Backbone size w/o insert (bp) 4281
- Total vector size (bp) 6771
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Vector typeMammalian Expression, AAV ; TALE
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRY2PHR-NLS-VP64
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SpeciesSynthetic
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Insert Size (bp)2490
- Promoter EF1-alpha
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Tag
/ Fusion Protein
- 2A_GFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer multiple (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA was a gift from Feng Zhang (Addgene plasmid # 48253 ; http://n2t.net/addgene:48253 ; RRID:Addgene_48253) -
For your References section:
Optical control of mammalian endogenous transcription and epigenetic states. Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, Platt RJ, Scott DA, Church GM, Zhang F. Nature. 2013 Jul 23. doi: 10.1038/nature12466. 10.1038/nature12466 PubMed 23877069
Map uploaded by the depositor.
