SypHer
(Plasmid #48250)

• Purpose: pH sensor
• Depositing Lab: Nicolas Demaurex
• Publication: Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84. doi: 10.1074/jbc.M110.159962. Epub 2011 Jan 11.

Backbone

• Vector backbone: pHyper-cyto
• Backbone manufacturer: Evrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SypHer
• Species: Synthetic
• Insert Size (bp): 1400
• Mutation: C199S
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pHyper was cloned by replacing EGFP with the Hyper sequence in the pEGFP-C1 vector (Clontech). To make pSypHer, the C199S mutation was made with the QuikChange II site-directed mutagenesis kit using the following oligos:
5′-agatggtcactctttgcgcgat-3′ (forward)
5′-atcgcgcaaagagtgaccatct-3′ (reverse)