pET29b-ClbP-S95A
(Plasmid
#48245)
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PurposeUntagged, full-length protein with active site mutation Serine95 to Alanine for in vivo peptidase assays
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET29b-(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5370
- Total vector size (bp) 6753
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameClbP
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SpeciesEscherichia coli CFT073
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Insert Size (bp)1521
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MutationChanged Serine 95 to Alanine
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GenBank IDNP_754344.1
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CGAGCGCATATGACAATAATGGAACACGTTAGCATTAAAAC
- 3′ sequencing primer TATTCTCGAGTTACTCATCGTCCCACTCCTTGTTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET29b-ClbP-S95A was a gift from Emily Balskus (Addgene plasmid # 48245 ; http://n2t.net/addgene:48245 ; RRID:Addgene_48245) -
For your References section:
A prodrug resistance mechanism is involved in colibactin biosynthesis and cytotoxicity. Brotherton CA, Balskus EP. J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. Epub 2013 Feb 20. 10.1021/ja312154m PubMed 23406518