pET29b-ClbP
(Plasmid #48244)

• Purpose: Untagged, full-length protein for in vivo peptidase assays
• Depositing Lab: Emily Balskus
• Publication: Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. Epub 2013 Feb 20.

Backbone

• Vector backbone: pET29b-(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5370
• Total vector size (bp): 6753
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ClbP
• Species: Escherichia coli CFT073
• Insert Size (bp): 1521
• GenBank ID: NP_754344.1
• Promoter: T7
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CGAGCGCATATGACAATAATGGAACACGTTAGCATTAAAAC
• 3′ sequencing primer: TATTCTCGAGTTACTCATCGTCCCACTCCTTGTTG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry