pULTRA-CNF
(Plasmid #48215)

• Purpose: highly efficient suppressor plasmid
• Depositing Lab: Peter Schultz
• Publication: Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-5.

Backbone

• Vector backbone: pULTRA
• Backbone manufacturer: Schultz lab
• Modifications to backbone: The Clodf13 replicon, spectinomycin resistance cassette and the lacI gene of pUltra were derived from pCDF-1b(Novagen). The T7 promoter/terminator elements in pCDF-1b were replaced with a tacI promoter sequence followed by a multiple-cloning site.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: polyspecific MJ tyrosyl synthetase
• Species: M. jannaschii
• Mutation: Y32L, L65V, F108W, Q109M, D158G, I159A
• Promoter: tacI

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: Tac promoter

Gene/Insert 2

• Gene/Insert name: tRNA
• Promoter: proK

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: SbfI (unknown if destroyed)
• 3′ cloning site: NcoI (unknown if destroyed)
• 5′ sequencing primer: na

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was also used in Chatterjee et al Biochemistry. 2013 Feb 27. PMID: 23379331