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Addgene

pET-29b-CylB
(Plasmid #48199)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48199 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5238
  • Total vector size (bp) 5562
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CylB
  • Alt name
    Acyl carrier protein
  • Species
    Cylindrospermum licheniforme
  • Insert Size (bp)
    324
  • GenBank ID
    AFV96136
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-29b-CylB was a gift from Emily Balskus (Addgene plasmid # 48199 ; http://n2t.net/addgene:48199 ; RRID:Addgene_48199)

  • For your References section:

    Cylindrocyclophane biosynthesis involves functionalization of an unactivated carbon center. Nakamura H, Hamer HA, Sirasani G, Balskus EP. J Am Chem Soc. 2012 Nov 14;134(45):18518-21. doi: 10.1021/ja308318p. Epub 2012 Nov 2. 10.1021/ja308318p PubMed 23106426
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