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Flag-ATXN1_V591A_L686E
(Plasmid #48191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA1.1/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4767
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ataxin1
  • Alt name
    ATXN1, ATX1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2671
  • Entrez Gene
    ATXN1 (a.k.a. ATX1, D6S504E, SCA1)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-ATXN1_V591A_L686E was a gift from Huda Zoghbi (Addgene plasmid # 48191 ; http://n2t.net/addgene:48191 ; RRID:Addgene_48191)

  • For your References section:

    Structural basis of protein complex formation and reconfiguration by polyglutamine disease protein Ataxin-1 and Capicua. Kim E, Lu HC, Zoghbi HY, Song JJ. Genes Dev. 2013 Mar 15;27(6):590-5. doi: 10.1101/gad.212068.112. 10.1101/gad.212068.112 PubMed 23512657
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