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psicheck-2-Hsdl2-mod
(Plasmid #48180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psicheck2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6273
  • Total vector size (bp) 6445
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5" UTR of HSDL2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    172
  • Mutation
    A/T MUTATION IN ATG OF UORF
  • GenBank ID
    NM_024255
  • Entrez Gene
    Hsdl2 (a.k.a. 2610207I16Rik)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Nhe1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psicheck-2-Hsdl2-mod was a gift from Vamsi Mootha (Addgene plasmid # 48180 ; http://n2t.net/addgene:48180 ; RRID:Addgene_48180)

  • For your References section:

    Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans. Calvo SE, Pagliarini DJ, Mootha VK. Proc Natl Acad Sci U S A. 2009 May 5;106(18):7507-12. doi: 10.1073/pnas.0810916106. Epub 2009 Apr 16. 10.1073/pnas.0810916106 PubMed 19372376
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