psicheck-2-Mut mod
(Plasmid
#48165)
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Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepsicheck2
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 6273
- Total vector size (bp) 6386
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5" UTR of Mut
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SpeciesM. musculus (mouse)
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Insert Size (bp)113
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MutationA/T mutation in the ATG of the uORF
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GenBank IDNM_008650
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Entrez GeneMut (a.k.a. D230010K02Rik, Mcm)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site Nhe1 (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psicheck-2-Mut mod was a gift from Vamsi Mootha (Addgene plasmid # 48165 ; http://n2t.net/addgene:48165 ; RRID:Addgene_48165) -
For your References section:
Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans. Calvo SE, Pagliarini DJ, Mootha VK. Proc Natl Acad Sci U S A. 2009 May 5;106(18):7507-12. doi: 10.1073/pnas.0810916106. Epub 2009 Apr 16. 10.1073/pnas.0810916106 PubMed 19372376
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/97/97/d9f8ffae-0f62-11e3-8786-003048dd6500.pdf.940x940_q85_autocrop.png)