pPSP6
(Plasmid
#48150)
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Purpose(Empty Backbone) Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMM1520
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Backbone manufacturerMalten et al., 2005
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Growth instructionsFor Bacillus megaterium: Tetracycline and 37 °C
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPSP6 was a gift from Dieter Jahn (Addgene plasmid # 48150 ; http://n2t.net/addgene:48150 ; RRID:Addgene_48150) -
For your References section:
Application of Escherichia coli phage K1E DNA-dependent RNA polymerase for in vitro RNA synthesis and in vivo protein production in Bacillus megaterium. Stammen S, Schuller F, Dietrich S, Gamer M, Biedendieck R, Jahn D. Appl Microbiol Biotechnol. 2010 Sep;88(2):529-39. doi: 10.1007/s00253-010-2732-y. Epub 2010 Jul 2. 10.1007/s00253-010-2732-y PubMed 20596705
Map uploaded by the depositor.
