pSpCas9n(BB)-2A-Puro (PX462) Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid
48141 with the following enzymes: Age I HF, NotI HF, EcoRV HF, Xbal, EcoRVHf + Xbal
Linearized plasmid is 9.2kb, double digest is 6.5kb and 2.8kb. Looks OK.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: AgeI HF, NotI HF, AgeI HF + NotI HF. The expected digest pattern was observed.
