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pSpCas9n(BB)-2A-GFP (PX461) Citations (136)

Originally described in: Genome engineering using the CRISPR-Cas9 system.
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed Journal

Articles Citing pSpCas9n(BB)-2A-GFP (PX461)

Articles
Genome editing using Cas9 nickases. Trevino AE, Zhang F. Methods Enzymol. 2014;546:161-74. doi: 10.1016/B978-0-12-801185-0.00008-8. PubMed
Splicing of many human genes involves sites embedded within introns. Kelly S, Georgomanolis T, Zirkel A, Diermeier S, O'Reilly D, Murphy S, Langst G, Cook PR, Papantonis A. Nucleic Acids Res. 2015 May 19;43(9):4721-32. doi: 10.1093/nar/gkv386. Epub 2015 Apr 20. PubMed
PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis. Nielsen CF, Huttner D, Bizard AH, Hirano S, Li TN, Palmai-Pallag T, Bjerregaard VA, Liu Y, Nigg EA, Wang LH, Hickson ID. Nat Commun. 2015 Dec 8;6:8962. doi: 10.1038/ncomms9962. PubMed
Ribonuclease H2 mutations induce a cGAS/STING-dependent innate immune response. Mackenzie KJ, Carroll P, Lettice L, Tarnauskaite Z, Reddy K, Dix F, Revuelta A, Abbondati E, Rigby RE, Rabe B, Kilanowski F, Grimes G, Fluteau A, Devenney PS, Hill RE, Reijns MA, Jackson AP. EMBO J. 2016 Apr 15;35(8):831-44. doi: 10.15252/embj.201593339. Epub 2016 Feb 22. PubMed
Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, Zoldos V. Nucleic Acids Res. 2016 Mar 11. pii: gkw159. PubMed

Associated Plasmids
CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Chiang TW, le Sage C, Larrieu D, Demir M, Jackson SP. Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356. PubMed

Associated Plasmids
Mesp1 controls the speed, polarity, and directionality of cardiovascular progenitor migration. Chiapparo G, Lin X, Lescroart F, Chabab S, Paulissen C, Pitisci L, Bondue A, Blanpain C. J Cell Biol. 2016 May 23;213(4):463-77. doi: 10.1083/jcb.201505082. Epub 2016 May 16. PubMed
Combination of the clustered regularly interspaced short palindromic repeats (CRISPR)-associated 9 technique with the piggybac transposon system for mouse in utero electroporation to study cortical development. Cheng M, Jin X, Mu L, Wang F, Li W, Zhong X, Liu X, Shen W, Liu Y, Zhou Y. J Neurosci Res. 2016 Jun 18. doi: 10.1002/jnr.23776. PubMed
Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Santos DP, Kiskinis E, Eggan K, Merkle FT. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15. PubMed
Recognition of enzymes lacking bound cofactor by protein quality control. Martinez-Limon A, Alriquet M, Lang WH, Calloni G, Wittig I, Vabulas RM. Proc Natl Acad Sci U S A. 2016 Oct 25;113(43):12156-12161. Epub 2016 Oct 12. PubMed
Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor-mediated inflammatory responses. Westphal A, Cheng W, Yu J, Grassl G, Krautkramer M, Holst O, Foger N, Lee KH. J Exp Med. 2017 Jan;214(1):227-244. doi: 10.1084/jem.20141461. Epub 2016 Nov 23. PubMed
Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro. Termglinchan V, Seeger T, Chen C, Wu JC, Karakikes I. Methods Mol Biol. 2017;1521:55-68. PubMed
Efficient and versatile CRISPR engineering of human neurons in culture to model neurological disorders. Shah RR, Cholewa-Waclaw J, Davies FC, Paton KM, Chaligne R, Heard E, Abbott CM, Bird AP. Wellcome Open Res. 2016 Nov 15;1:13. PubMed
Loss of RMI2 Increases Genome Instability and Causes a Bloom-Like Syndrome. Hudson DF, Amor DJ, Boys A, Butler K, Williams L, Zhang T, Kalitsis P. PLoS Genet. 2016 Dec 15;12(12):e1006483. doi: 10.1371/journal.pgen.1006483. eCollection 2016 Dec. PubMed
Small GTPase Rab8a-recruited Phosphatidylinositol 3-Kinase gamma Regulates Signaling and Cytokine Outputs from Endosomal Toll-like Receptors. Wall AA, Luo L, Hung Y, Tong SJ, Condon ND, Blumenthal A, Sweet MJ, Stow JL. J Biol Chem. 2017 Mar 17;292(11):4411-4422. doi: 10.1074/jbc.M116.766337. Epub 2017 Jan 27. PubMed
Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects. Gao Y, Wu H, Wang Y, Liu X, Chen L, Li Q, Cui C, Liu X, Zhang J, Zhang Y. Genome Biol. 2017 Feb 1;18(1):13. doi: 10.1186/s13059-016-1144-4. PubMed
The non-coding variant rs1800734 enhances DCLK3 expression through long-range interaction and promotes colorectal cancer progression. Liu NQ, Ter Huurne M, Nguyen LN, Peng T, Wang SY, Studd JB, Joshi O, Ongen H, Bramsen JB, Yan J, Andersen CL, Taipale J, Dermitzakis ET, Houlston RS, Hubner NC, Stunnenberg HG. Nat Commun. 2017 Feb 14;8:14418. doi: 10.1038/ncomms14418. PubMed
Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse. Ashdown GW, Burn GL, Williamson DJ, Pandzic E, Peters R, Holden M, Ewers H, Shao L, Wiseman PW, Owen DM. Biophys J. 2017 Apr 25;112(8):1703-1713. doi: 10.1016/j.bpj.2017.01.038. PubMed
Cancer cachexia associates with a systemic autophagy-inducing activity mimicked by cancer cell-derived IL-6 trans-signaling. Pettersen K, Andersen S, Degen S, Tadini V, Grosjean J, Hatakeyama S, Tesfahun AN, Moestue S, Kim J, Nonstad U, Romundstad PR, Skorpen F, Sorhaug S, Amundsen T, Gronberg BH, Strasser F, Stephens N, Hoem D, Molven A, Kaasa S, Fearon K, Jacobi C, Bjorkoy G. Sci Rep. 2017 May 17;7(1):2046. doi: 10.1038/s41598-017-02088-2. PubMed
MLK3 regulates FRA-1 and MMPs to drive invasion and transendothelial migration in triple-negative breast cancer cells. Rattanasinchai C, Llewellyn BJ, Conrad SE, Gallo KA. Oncogenesis. 2017 Jun 12;6(6):e345. doi: 10.1038/oncsis.2017.44. PubMed
DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes. Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW. Cell. 2017 Aug 24;170(5):956-972.e23. doi: 10.1016/j.cell.2017.07.038. PubMed

Associated Plasmids
N6-methyladenosine demethylase FTO targets pre-mRNAs and regulates alternative splicing and 3'-end processing. Bartosovic M, Molares HC, Gregorova P, Hrossova D, Kudla G, Vanacova S. Nucleic Acids Res. 2017 Nov 2;45(19):11356-11370. doi: 10.1093/nar/gkx778. PubMed
CHIP as a membrane-shuttling proteostasis sensor. Kopp Y, Lang WH, Schuster TB, Martinez-Limon A, Hofbauer HF, Ernst R, Calloni G, Vabulas RM. Elife. 2017 Nov 1;6. doi: 10.7554/eLife.29388. PubMed
Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression. Stevenson NL, Bergen DJM, Skinner REH, Kague E, Martin-Silverstone E, Robson Brown KA, Hammond CL, Stephens DJ. J Cell Sci. 2017 Dec 15;130(24):4132-4143. doi: 10.1242/jcs.212308. Epub 2017 Nov 1. PubMed
p63 and p73 repress CXCR5 chemokine receptor gene expression in p53-deficient MCF-7 breast cancer cells during genotoxic stress. Mitkin NA, Muratova AM, Sharonov GV, Korneev KV, Sviriaeva EN, Mazurov D, Schwartz AM, Kuprash DV. Biochim Biophys Acta Gene Regul Mech. 2017 Dec;1860(12):1169-1178. doi: 10.1016/j.bbagrm.2017.10.003. Epub 2017 Nov 6. PubMed
Evaluation and validation of commercial antibodies for the detection of Shb. Vanli G, Cuesta-Marban A, Widmann C. PLoS One. 2017 Dec 1;12(12):e0188311. doi: 10.1371/journal.pone.0188311. eCollection 2017. PubMed
Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. Adikusuma F, Pfitzner C, Thomas PQ. PLoS One. 2017 Dec 6;12(12):e0187236. doi: 10.1371/journal.pone.0187236. eCollection 2017. PubMed

Associated Plasmids
Generation of two H1 hESC sublines carrying a heterozygous and homozygous knock-out of RB1. Steenpass L. Stem Cell Res. 2017 Dec;25:270-273. doi: 10.1016/j.scr.2017.07.005. Epub 2017 Jul 11. PubMed
Gene editing rescue of a novel MPL mutant associated with congenital amegakaryocytic thrombocytopenia. Cleyrat C, Girard R, Choi EH, Jeziorski E, Lavabre-Bertrand T, Hermouet S, Carillo S, Wilson BS. Blood Adv. 2017 Sep 22;1(21):1815-1826. doi: 10.1182/bloodadvances.2016002915. eCollection 2017 Sep 26. PubMed
BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange-like syndrome. Olley G, Ansari M, Bengani H, Grimes GR, Rhodes J, von Kriegsheim A, Blatnik A, Stewart FJ, Wakeling E, Carroll N, Ross A, Park SM, Bickmore WA, Pradeepa MM, FitzPatrick DR. Nat Genet. 2018 Mar;50(3):329-332. doi: 10.1038/s41588-018-0042-y. Epub 2018 Jan 29. PubMed
Karyopherin Alpha 6 Is Required for Replication of Porcine Reproductive and Respiratory Syndrome Virus and Zika Virus. Yang L, Wang R, Yang S, Ma Z, Lin S, Nan Y, Li Q, Tang Q, Zhang YJ. J Virol. 2018 Apr 13;92(9). pii: JVI.00072-18. doi: 10.1128/JVI.00072-18. Print 2018 May 1. PubMed
Regulation of RUNX1 dosage is crucial for efficient blood formation from hemogenic endothelium. Lie-A-Ling M, Marinopoulou E, Lilly AJ, Challinor M, Patel R, Lancrin C, Kouskoff V, Lacaud G. Development. 2018 Mar 12;145(5). pii: 145/5/dev149419. doi: 10.1242/dev.149419. PubMed
Voltage gating of mechanosensitive PIEZO channels. Moroni M, Servin-Vences MR, Fleischer R, Sanchez-Carranza O, Lewin GR. Nat Commun. 2018 Mar 15;9(1):1096. doi: 10.1038/s41467-018-03502-7. PubMed
STING-Dependent Interferon-lambda1 Induction in HT29 Cells, a Human Colorectal Cancer Cell Line, After Gamma-Radiation. Chen J, Markelc B, Kaeppler J, Ogundipe VML, Cao Y, McKenna WG, Muschel RJ. Int J Radiat Oncol Biol Phys. 2018 May 1;101(1):97-106. doi: 10.1016/j.ijrobp.2018.01.091. Epub 2018 Feb 3. PubMed
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation. Corsini NS, Peer AM, Moeseneder P, Roiuk M, Burkard TR, Theussl HC, Moll I, Knoblich JA. Cell Stem Cell. 2018 Apr 5;22(4):543-558.e12. doi: 10.1016/j.stem.2018.03.002. PubMed
Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells. Riesenberg S, Maricic T. Nat Commun. 2018 Jun 4;9(1):2164. doi: 10.1038/s41467-018-04609-7. PubMed
Oncogenic BRAFV600E drives expression of MGL ligands in the colorectal cancer cell line HT29 through N-acetylgalactosamine-transferase 3. Sahasrabudhe NM, Lenos K, van der Horst JC, Rodriguez E, van Vliet SJ. Biol Chem. 2018 Jun 27;399(7):649-659. doi: 10.1515/hsz-2018-0120. PubMed
Adipocyte-Derived Lipids Mediate Melanoma Progression via FATP Proteins. Zhang M, Di Martino JS, Bowman RL, Campbell NR, Baksh SC, Simon-Vermot T, Kim IS, Haldeman P, Mondal C, Yong-Gonzales V, Abu-Akeel M, Merghoub T, Jones DR, Zhu XG, Arora A, Ariyan CE, Birsoy K, Wolchok JD, Panageas KS, Hollmann T, Bravo-Cordero JJ, White RM. Cancer Discov. 2018 Aug;8(8):1006-1025. doi: 10.1158/2159-8290.CD-17-1371. Epub 2018 Jun 14. PubMed
RNase H2, mutated in Aicardi-Goutieres syndrome, promotes LINE-1 retrotransposition. Benitez-Guijarro M, Lopez-Ruiz C, Tarnauskaite Z, Murina O, Mian Mohammad M, Williams TC, Fluteau A, Sanchez L, Vilar-Astasio R, Garcia-Canadas M, Cano D, Kempen MH, Sanchez-Pozo A, Heras SR, Jackson AP, Reijns MA, Garcia-Perez JL. EMBO J. 2018 Aug 1;37(15). pii: embj.201798506. doi: 10.15252/embj.201798506. Epub 2018 Jun 29. PubMed

Associated Plasmids
CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Zimmermann M, Murina O, Reijns MAM, Agathanggelou A, Challis R, Tarnauskaite Z, Muir M, Fluteau A, Aregger M, McEwan A, Yuan W, Clarke M, Lambros MB, Paneesha S, Moss P, Chandrashekhar M, Angers S, Moffat J, Brunton VG, Hart T, de Bono J, Stankovic T, Jackson AP, Durocher D. Nature. 2018 Jul;559(7713):285-289. doi: 10.1038/s41586-018-0291-z. Epub 2018 Jul 4. PubMed
GRHL2-Dependent Enhancer Switching Maintains a Pluripotent Stem Cell Transcriptional Subnetwork after Exit from Naive Pluripotency. Chen AF, Liu AJ, Krishnakumar R, Freimer JW, DeVeale B, Blelloch R. Cell Stem Cell. 2018 Aug 2;23(2):226-238.e4. doi: 10.1016/j.stem.2018.06.005. Epub 2018 Jul 12. PubMed
Tumor endothelial marker 8 promotes cancer progression and metastasis. Hoye AM, Tolstrup SD, Horton ER, Nicolau M, Frost H, Woo JH, Mauldin JP, Frankel AE, Cox TR, Erler JT. Oncotarget. 2018 Jul 10;9(53):30173-30188. doi: 10.18632/oncotarget.25734. eCollection 2018 Jul 10. PubMed
GFAP Mutations in Astrocytes Impair Oligodendrocyte Progenitor Proliferation and Myelination in an hiPSC Model of Alexander Disease. Li L, Tian E, Chen X, Chao J, Klein J, Qu Q, Sun G, Sun G, Huang Y, Warden CD, Ye P, Feng L, Li X, Cui Q, Sultan A, Douvaras P, Fossati V, Sanjana NE, Riggs AD, Shi Y. Cell Stem Cell. 2018 Aug 2;23(2):239-251.e6. doi: 10.1016/j.stem.2018.07.009. PubMed
Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to Pluripotency. Hernandez C, Wang Z, Ramazanov B, Tang Y, Mehta S, Dambrot C, Lee YW, Tessema K, Kumar I, Astudillo M, Neubert TA, Guo S, Ivanova NB. Cell Stem Cell. 2018 Sep 6;23(3):396-411.e8. doi: 10.1016/j.stem.2018.08.001. Epub 2018 Aug 23. PubMed
ATRX loss induces multiple hallmarks of the alternative lengthening of telomeres (ALT) phenotype in human glioma cell lines in a cell line-specific manner. Brosnan-Cashman JA, Yuan M, Graham MK, Rizzo AJ, Myers KM, Davis C, Zhang R, Esopi DM, Raabe EH, Eberhart CG, Heaphy CM, Meeker AK. PLoS One. 2018 Sep 18;13(9):e0204159. doi: 10.1371/journal.pone.0204159. eCollection 2018. PubMed
Acidic Bile Salts Induce Epithelial to Mesenchymal Transition via VEGF Signaling in Non-Neoplastic Barrett's Cells. Zhang Q, Agoston AT, Pham TH, Zhang W, Zhang X, Huo X, Peng S, Bajpai M, Das K, Odze RD, Spechler SJ, Souza RF. Gastroenterology. 2019 Jan;156(1):130-144.e10. doi: 10.1053/j.gastro.2018.09.046. Epub 2018 Sep 27. PubMed
Universal Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells for Cellularization of a Corneal Scaffold. Yang J, Park JW, Zheng D, Xu RH. Transl Vis Sci Technol. 2018 Oct 10;7(5):23. doi: 10.1167/tvst.7.5.23. eCollection 2018 Sep. PubMed
Loss of function mutations in KIF14 cause severe microcephaly and kidney development defects in humans and zebrafish. Reilly ML, Stokman MF, Magry V, Jeanpierre C, Alves M, Paydar M, Hellinga J, Delous M, Pouly D, Failler M, Martinovic J, Loeuillet L, Leroy B, Tantau J, Roume J, Evans CG, Shan X, Filges I, Allingham JS, Kwok BH, Saunier S, Giles RH, Benmerah A. Hum Mol Genet. 2018 Nov 1. pii: 5154958. doi: 10.1093/hmg/ddy381. PubMed
Inherent DNA-binding specificities of the HIF-1alpha and HIF-2alpha transcription factors in chromatin. Smythies JA, Sun M, Masson N, Salama R, Simpson PD, Murray E, Neumann V, Cockman ME, Choudhry H, Ratcliffe PJ, Mole DR. EMBO Rep. 2019 Jan;20(1). pii: embr.201846401. doi: 10.15252/embr.201846401. Epub 2018 Nov 14. PubMed
Gain-of-function DNMT3A mutations cause microcephalic dwarfism and hypermethylation of Polycomb-regulated regions. Heyn P, Logan CV, Fluteau A, Challis RC, Auchynnikava T, Martin CA, Marsh JA, Taglini F, Kilanowski F, Parry DA, Cormier-Daire V, Fong CT, Gibson K, Hwa V, Ibanez L, Robertson SP, Sebastiani G, Rappsilber J, Allshire RC, Reijns MAM, Dauber A, Sproul D, Jackson AP. Nat Genet. 2019 Jan;51(1):96-105. doi: 10.1038/s41588-018-0274-x. Epub 2018 Nov 26. PubMed
The genetic intractability of Symbiodinium microadriaticum to standard algal transformation methods. Chen JE, Barbrook AC, Cui G, Howe CJ, Aranda M. PLoS One. 2019 Feb 19;14(2):e0211936. doi: 10.1371/journal.pone.0211936. eCollection 2019. PubMed
Kindlin-2 links mechano-environment to proline synthesis and tumor growth. Guo L, Cui C, Zhang K, Wang J, Wang Y, Lu Y, Chen K, Yuan J, Xiao G, Tang B, Sun Y, Wu C. Nat Commun. 2019 Feb 19;10(1):845. doi: 10.1038/s41467-019-08772-3. PubMed
Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Back S, Necarsulmer J, Whitaker LR, Coke LM, Koivula P, Heathward EJ, Fortuno LV, Zhang Y, Yeh CG, Baldwin HA, Spencer MD, Mejias-Aponte CA, Pickel J, Hoffman AF, Spivak CE, Lupica CR, Underhill SM, Amara SG, Domanskyi A, Anttila JE, Airavaara M, Hope BT, Hamra FK, Richie CT, Harvey BK. Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5. doi: 10.1016/j.neuron.2019.01.035. PubMed

Associated Plasmids
Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. Baron DM, Matheny T, Lin YC, Leszyk JD, Kenna K, Gall KV, Santos DP, Tischbein M, Funes S, Hayward LJ, Kiskinis E, Landers JE, Parker R, Shaffer SA, Bosco DA. Hum Mol Genet. 2019 Jul 1;28(13):2143-2160. doi: 10.1093/hmg/ddz048. PubMed
Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X. Colognori D, Sunwoo H, Kriz AJ, Wang CY, Lee JT. Mol Cell. 2019 Apr 4;74(1):101-117.e10. doi: 10.1016/j.molcel.2019.01.015. Epub 2019 Feb 28. PubMed
CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations. Cullot G, Boutin J, Toutain J, Prat F, Pennamen P, Rooryck C, Teichmann M, Rousseau E, Lamrissi-Garcia I, Guyonnet-Duperat V, Bibeyran A, Lalanne M, Prouzet-Mauleon V, Turcq B, Ged C, Blouin JM, Richard E, Dabernat S, Moreau-Gaudry F, Bedel A. Nat Commun. 2019 Mar 8;10(1):1136. doi: 10.1038/s41467-019-09006-2. PubMed
The tumor suppressor Sef is a scaffold for the classical NF-kappaB/RELA:P50 signaling module. Korsensky L, Haif S, Heller R, Rabinovitz S, Haddad-Halloun J, Dahan N, Ron D. Cell Signal. 2019 Jul;59:110-121. doi: 10.1016/j.cellsig.2019.01.009. Epub 2019 Mar 9. PubMed
H3K27M induces defective chromatin spread of PRC2-mediated repressive H3K27me2/me3 and is essential for glioma tumorigenesis. Harutyunyan AS, Krug B, Chen H, Papillon-Cavanagh S, Zeinieh M, De Jay N, Deshmukh S, Chen CCL, Belle J, Mikael LG, Marchione DM, Li R, Nikbakht H, Hu B, Cagnone G, Cheung WA, Mohammadnia A, Bechet D, Faury D, McConechy MK, Pathania M, Jain SU, Ellezam B, Weil AG, Montpetit A, Salomoni P, Pastinen T, Lu C, Lewis PW, Garcia BA, Kleinman CL, Jabado N, Majewski J. Nat Commun. 2019 Mar 19;10(1):1262. doi: 10.1038/s41467-019-09140-x. PubMed
Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns. Alateeq S, Ovchinnikov D, Tracey T, Whitworth D, Al-Rubaish A, Al-Ali A, Wolvetang E. APL Bioeng. 2018 Dec 3;2(4):046103. doi: 10.1063/1.5048625. eCollection 2018 Dec. PubMed
SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, Stewart A, Svejstrup JQ. Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 10.1016/j.cell.2019.04.038. PubMed

Associated Plasmids
Species specific differences in use of ANP32 proteins by influenza A virus. Long JS, Idoko-Akoh A, Mistry B, Goldhill D, Staller E, Schreyer J, Ross C, Goodbourn S, Shelton H, Skinner MA, Sang H, McGrew MJ, Barclay W. Elife. 2019 Jun 4;8. pii: 45066. doi: 10.7554/eLife.45066. PubMed
PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments. Wang CY, Colognori D, Sunwoo H, Wang D, Lee JT. Nat Commun. 2019 Jul 3;10(1):2950. doi: 10.1038/s41467-019-10755-3. PubMed
A novel mutation in SPART gene causes a severe neurodevelopmental delay due to mitochondrial dysfunction with complex I impairments and altered pyruvate metabolism. Diquigiovanni C, Bergamini C, Diaz R, Liparulo I, Bianco F, Masin L, Baldassarro VA, Rizzardi N, Tranchina A, Buscherini F, Wischmeijer A, Pippucci T, Scarano E, Cordelli DM, Fato R, Seri M, Paracchini S, Bonora E. FASEB J. 2019 Oct;33(10):11284-11302. doi: 10.1096/fj.201802722R. Epub 2019 Aug 7. PubMed
Transcriptional profiling of breast cancer-associated lymphatic vessels reveals VCAM-1 as regulator of lymphatic invasion and permeability. Dieterich LC, Kapaklikaya K, Cetintas T, Proulx ST, Commerford CD, Ikenberg K, Bachmann SB, Scholl J, Detmar M. Int J Cancer. 2019 Nov 15;145(10):2804-2815. doi: 10.1002/ijc.32594. Epub 2019 Aug 8. PubMed
Myosin-II mediated traction forces evoke localized Piezo1-dependent Ca(2+) flickers. Ellefsen KL, Holt JR, Chang AC, Nourse JL, Arulmoli J, Mekhdjian AH, Abuwarda H, Tombola F, Flanagan LA, Dunn AR, Parker I, Pathak MM. Commun Biol. 2019 Aug 7;2:298. doi: 10.1038/s42003-019-0514-3. eCollection 2019. PubMed
Expanding C-T base editing toolkit with diversified cytidine deaminases. Cheng TL, Li S, Yuan B, Wang X, Zhou W, Qiu Z. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. PubMed

Associated Plasmids
Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state. Menges J, Cremanns M, Steenpass L. Stem Cell Res. 2019 Aug;39:101517. doi: 10.1016/j.scr.2019.101517. Epub 2019 Jul 29. PubMed
Type I IFN protects cancer cells from CD8+ T cell-mediated cytotoxicity after radiation. Chen J, Cao Y, Markelc B, Kaeppler J, Vermeer JA, Muschel RJ. J Clin Invest. 2019 Oct 1;129(10):4224-4238. doi: 10.1172/JCI127458. PubMed
Characterizing CDK8/19 Inhibitors through a NFkappaB-Dependent Cell-Based Assay. Li J, Ji H, Porter DC, Broude EV, Roninson IB, Chen M. Cells. 2019 Oct 6;8(10). pii: cells8101208. doi: 10.3390/cells8101208. PubMed
Functional Loss of ATRX and TERC Activates Alternative Lengthening of Telomeres (ALT) in LAPC4 Prostate Cancer Cells. Graham MK, Kim J, Da J, Brosnan-Cashman JA, Rizzo A, Baena Del Valle JA, Chia L, Rubenstein M, Davis C, Zheng Q, Cope L, Considine M, Haffner MC, De Marzo AM, Meeker AK, Heaphy CM. Mol Cancer Res. 2019 Dec;17(12):2480-2491. doi: 10.1158/1541-7786.MCR-19-0654. Epub 2019 Oct 14. PubMed
The RNA polymerase III repressor MAF1 is regulated by ubiquitin-dependent proteasome degradation and modulates cancer drug resistance and apoptosis. Wang X, Rusin A, Walkey CJ, Lin JJ, Johnson DL. J Biol Chem. 2019 Dec 13;294(50):19255-19268. doi: 10.1074/jbc.RA119.008849. Epub 2019 Oct 23. PubMed
Loss of TOP3B leads to increased R-loop formation and genome instability. Zhang T, Wallis M, Petrovic V, Challis J, Kalitsis P, Hudson DF. Open Biol. 2019 Dec;9(12):190222. doi: 10.1098/rsob.190222. Epub 2019 Dec 4. PubMed
ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress. Park SH, Kang N, Song E, Wie M, Lee EA, Hwang S, Lee D, Ra JS, Park IB, Park J, Kang S, Park JH, Hohng S, Lee KY, Myung K. Nat Commun. 2019 Dec 16;10(1):5718. doi: 10.1038/s41467-019-13667-4. PubMed
Generation of new hepatocyte-like in vitro models better resembling human lipid metabolism. Pramfalk C, Jakobsson T, Verzijl CRC, Minniti ME, Obensa C, Ripamonti F, Olin M, Pedrelli M, Eriksson M, Parini P. Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Jun;1865(6):158659. doi: 10.1016/j.bbalip.2020.158659. Epub 2020 Feb 11. PubMed
TMEM87a/Elkin1, a component of a novel mechanoelectrical transduction pathway, modulates melanoma adhesion and migration. Patkunarajah A, Stear JH, Moroni M, Schroeter L, Blaszkiewicz J, Tearle JL, Cox CD, Furst C, Sanchez-Carranza O, Ocana Fernandez MDA, Fleischer R, Eravci M, Weise C, Martinac B, Biro M, Lewin GR, Poole K. Elife. 2020 Apr 1;9. pii: 53308. doi: 10.7554/eLife.53308. PubMed
TrkB-expressing paraventricular hypothalamic neurons suppress appetite through multiple neurocircuits. An JJ, Kinney CE, Tan JW, Liao GY, Kremer EJ, Xu B. Nat Commun. 2020 Apr 7;11(1):1729. doi: 10.1038/s41467-020-15537-w. PubMed

Associated Plasmids
Enhancement of homology-directed repair with chromatin donor templates in cells. Cruz-Becerra G, Kadonaga JT. Elife. 2020 Apr 28;9. pii: 55780. doi: 10.7554/eLife.55780. PubMed
Parkin ubiquitinates phosphoglycerate dehydrogenase to suppress serine synthesis and tumor progression. Liu J, Zhang C, Wu H, Sun XX, Li Y, Huang S, Yue X, Lu SE, Shen Z, Su X, White E, Haffty BG, Hu W, Feng Z. J Clin Invest. 2020 Jun 1;130(6):3253-3269. doi: 10.1172/JCI132876. PubMed
Biallelic loss-of-function variants in TBC1D2B cause a neurodevelopmental disorder with seizures and gingival overgrowth. Harms FL, Parthasarathy P, Zorndt D, Alawi M, Fuchs S, Halliday BJ, McKeown C, Sampaio H, Radhakrishnan N, Radhakrishnan SK, Gorce M, Navet B, Ziegler A, Sachdev R, Robertson SP, Nampoothiri S, Kutsche K. Hum Mutat. 2020 Sep;41(9):1645-1661. doi: 10.1002/humu.24071. Epub 2020 Jul 15. PubMed
Unexpected Mutations by CRISPR-Cas9 CTG Repeat Excision in Myotonic Dystrophy and Use of CRISPR Interference as an Alternative Approach. Ikeda M, Taniguchi-Ikeda M, Kato T, Shinkai Y, Tanaka S, Hagiwara H, Sasaki N, Masaki T, Matsumura K, Sonoo M, Kurahashi H, Saito F. Mol Ther Methods Clin Dev. 2020 May 22;18:131-144. doi: 10.1016/j.omtm.2020.05.024. eCollection 2020 Sep 11. PubMed
The ZGRF1 Helicase Promotes Recombinational Repair of Replication-Blocking DNA Damage in Human Cells. Brannvoll A, Xue X, Kwon Y, Kompocholi S, Simonsen AKW, Viswalingam KS, Gonzalez L, Hickson ID, Oestergaard VH, Mankouri HW, Sung P, Lisby M. Cell Rep. 2020 Jul 7;32(1):107849. doi: 10.1016/j.celrep.2020.107849. PubMed
Structural basis of human full-length kindlin-3 homotrimer in an auto-inhibited state. Bu W, Levitskaya Z, Loh ZY, Jin S, Basu S, Ero R, Yan X, Wang M, Ngan SFC, Sze SK, Tan SM, Gao YG. PLoS Biol. 2020 Jul 9;18(7):e3000755. doi: 10.1371/journal.pbio.3000755. eCollection 2020 Jul. PubMed
Generation of OCT4-EGFP, NANOG-tdTomato dual reporter human induced pluripotent stem cell line, KKUi001-A, using the CRISPR/Cas9 system. Lee M, Choi NY, Park S, Bang JS, Lee Y, Jeong D, Ham S, Lim S, Kim KH, Ko K. Stem Cell Res. 2020 Oct;48:101943. doi: 10.1016/j.scr.2020.101943. Epub 2020 Aug 3. PubMed
Asbestos conceives Fe(II)-dependent mutagenic stromal milieu through ceaseless macrophage ferroptosis and beta-catenin induction in mesothelium. Ito F, Yanatori I, Maeda Y, Nimura K, Ito S, Hirayama T, Nagasawa H, Kohyama N, Okazaki Y, Akatsuka S, Toyokuni S. Redox Biol. 2020 Sep;36:101616. doi: 10.1016/j.redox.2020.101616. Epub 2020 Jun 24. PubMed
Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore. Pinzaru AM, Kareh M, Lamm N, Lazzerini-Denchi E, Cesare AJ, Sfeir A. Genes Dev. 2020 Dec 1;34(23-24):1619-1636. doi: 10.1101/gad.337287.120. Epub 2020 Oct 29. PubMed

Associated Plasmids
Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination. Li S, Yuan B, Cao J, Chen J, Chen J, Qiu J, Zhao XM, Wang X, Qiu Z, Cheng TL. Nat Commun. 2020 Nov 17;11(1):5827. doi: 10.1038/s41467-020-19730-9. PubMed
miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1. Kato M, Abdollahi M, Tunduguru R, Tsark W, Chen Z, Wu X, Wang J, Chen ZB, Lin FM, Lanting L, Wang M, Huss J, Fueger PT, Chan D, Natarajan R. Commun Biol. 2021 Jan 4;4(1):30. doi: 10.1038/s42003-020-01516-w. PubMed
Pannexin 1 mutation found in melanoma tumor reduces phosphorylation, glycosylation, and trafficking of the channel-forming protein. Nouri-Nejad D, O'Donnell BL, Patil CS, Sanchez-Pupo RE, Johnston D, Sayedyahossein S, Jurcic K, Lau R, Gyenis L, Litchfield DW, Jackson MF, Gloor GB, Penuela S. Mol Biol Cell. 2021 Mar 1;32(5):376-390. doi: 10.1091/mbc.E19-10-0585. Epub 2021 Jan 6. PubMed
Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases. Ye T, Duan Y, Tsang HWS, Xu H, Chen Y, Cao H, Chen Y, Fu AKY, Ip NY. Commun Biol. 2021 Feb 12;4(1):195. doi: 10.1038/s42003-021-01722-0. PubMed
Advanced single-cell pooled CRISPR screening identifies C19orf53 required for cell proliferation based on mTORC1 regulators. Tang Y, Liao S, Liu G, Xiong X, Liu H, Li F, Tan Z, Kong X, Yin Y, Tan B. Cell Biol Toxicol. 2021 Feb 13. pii: 10.1007/s10565-021-09586-0. doi: 10.1007/s10565-021-09586-0. PubMed
Pannexin 1 binds beta-catenin to modulate melanoma cell growth and metabolism. Sayedyahossein S, Huang K, Li Z, Zhang C, Kozlov AM, Johnston D, Nouri-Nejad D, Dagnino L, Betts DH, Sacks DB, Penuela S. J Biol Chem. 2021 Jan-Jun;296:100478. doi: 10.1016/j.jbc.2021.100478. Epub 2021 Feb 26. PubMed
Modulation of APLNR Signaling Is Required during the Development and Maintenance of the Hematopoietic System. Jackson M, Fidanza A, Taylor AH, Rybtsov S, Axton R, Kydonaki M, Meek S, Burdon T, Medvinsky A, Forrester LM. Stem Cell Reports. 2021 Apr 13;16(4):727-740. doi: 10.1016/j.stemcr.2021.02.003. Epub 2021 Mar 4. PubMed
Dysregulation of mitochondria-lysosome contacts by GBA1 dysfunction in dopaminergic neuronal models of Parkinson's disease. Kim S, Wong YC, Gao F, Krainc D. Nat Commun. 2021 Mar 22;12(1):1807. doi: 10.1038/s41467-021-22113-3. PubMed
Optogenetics-based localization of talin to the plasma membrane promotes activation of beta3 integrins. Liao Z, Gingras AR, Lagarrigue F, Ginsberg MH, Shattil SJ. J Biol Chem. 2021 Jan-Jun;296:100675. doi: 10.1016/j.jbc.2021.100675. Epub 2021 Apr 16. PubMed
Autophagy Inhibitors Do Not Restore Peroxisomal Functions in Cells With the Most Common Peroxisome Biogenesis Defect. Klouwer FCC, Falkenberg KD, Ofman R, Koster J, van Gent D, Ferdinandusse S, Wanders RJA, Waterham HR. Front Cell Dev Biol. 2021 Apr 1;9:661298. doi: 10.3389/fcell.2021.661298. eCollection 2021. PubMed
Nephrotic-syndrome-associated mutation of KANK2 induces pathologic binding competition with physiological interactor KIF21A. Xu Y, Guo C, Pan W, Zhao C, Ding Y, Xie X, Wei Z, Sun Y, Yu C. J Biol Chem. 2021 Aug;297(2):100958. doi: 10.1016/j.jbc.2021.100958. Epub 2021 Jul 16. PubMed
Dual-Specificity Phosphatase 9 Regulates Cellular Proliferation and Predicts Recurrence After Surgery in Hepatocellular Carcinoma. Chen K, Gorgen A, Ding A, Du L, Jiang K, Ding Y, Sapisochin G, Ghanekar A. Hepatol Commun. 2021 Mar 11;5(7):1310-1328. doi: 10.1002/hep4.1701. eCollection 2021 Jul. PubMed
Phosphorylation of OGFOD1 by Cell Cycle-Dependent Kinase 7/9 Enhances the Transcriptional Activity of RNA Polymerase II in Breast Cancer Cells. Lee HT, Lee IH, Kim JH, Lee S, Kwak S, Suh MY, Hwang IY, Kang BG, Cha SS, Lee BI, Lee SE, Choi J, Roe JS, Cho EJ, Youn HD. Cancers (Basel). 2021 Jul 8;13(14). pii: cancers13143418. doi: 10.3390/cancers13143418. PubMed
Targeting the Highly Expressed microRNA miR-146b with CRISPR/Cas9n Gene Editing System in Thyroid Cancer. Santa-Inez DC, Fuziwara CS, Saito KC, Kimura ET. Int J Mol Sci. 2021 Jul 27;22(15). pii: ijms22157992. doi: 10.3390/ijms22157992. PubMed
Cortical overgrowth in a preclinical forebrain organoid model of CNTNAP2-associated autism spectrum disorder. de Jong JO, Llapashtica C, Genestine M, Strauss K, Provenzano F, Sun Y, Zhu H, Cortese GP, Brundu F, Brigatti KW, Corneo B, Migliori B, Tomer R, Kushner SA, Kellendonk C, Javitch JA, Xu B, Markx S. Nat Commun. 2021 Sep 1;12(1):4087. doi: 10.1038/s41467-021-24358-4. PubMed
Multiplexed Simian Immunodeficiency Virus-Specific Paired RNA-Guided Cas9 Nickases Inactivate Proviral DNA. Smith LM, Ladner JT, Hodara VL, Parodi LM, Harris RA, Callery JE, Lai Z, Zou Y, Raveedran M, Rogers J, Giavedoni LD. J Virol. 2021 Nov 9;95(23):e0088221. doi: 10.1128/JVI.00882-21. Epub 2021 Sep 22. PubMed
Timely termination of repair DNA synthesis by ATAD5 is important in oxidative DNA damage-induced single-strand break repair. Park SH, Kim Y, Ra JS, Wie MW, Kang MS, Kang S, Myung K, Lee KY. Nucleic Acids Res. 2021 Nov 18;49(20):11746-11764. doi: 10.1093/nar/gkab999. PubMed
Identification of ASCL1 as a determinant for human iPSC-derived dopaminergic neurons. Earley AM, Burbulla LF, Krainc D, Awatramani R. Sci Rep. 2021 Nov 15;11(1):22257. doi: 10.1038/s41598-021-01366-4. PubMed
Experimental strategies to achieve efficient targeted knock-in via tandem paired nicking. Rahman ML, Hyodo T, Karnan S, Ota A, Hasan MN, Mihara Y, Wahiduzzaman M, Tsuzuki S, Hosokawa Y, Konishi H. Sci Rep. 2021 Nov 19;11(1):22627. doi: 10.1038/s41598-021-01978-w. PubMed
CRISPR/Cas9-mediated demethylation of FOXP3-TSDR toward Treg-characteristic programming of Jurkat T cells. Wilk C, Effenberg L, Abberger H, Steenpass L, Hansen W, Zeschnigk M, Kirschning C, Buer J, Kehrmann J. Cell Immunol. 2022 Jan;371:104471. doi: 10.1016/j.cellimm.2021.104471. Epub 2021 Dec 20. PubMed
CRISPR-Mediated Genomic Addition to CPS1 Deficient iPSCs is Insufficient to Restore Nitrogen Homeostasis. Nitzahn M, Truong B, Khoja S, Vega-Crespo A, Le C, Eliav A, Makris G, Pyle AD, Haberle J, Lipshutz GS. Yale J Biol Med. 2021 Dec 29;94(4):545-557. eCollection 2021 Dec. PubMed
Engineering of human mesenchymal stem cells resistant to multiple natural killer subtypes. Zheng D, Wang X, Zhang Z, Li E, Yeung C, Borkar R, Qin G, Wu Y, Xu RH. Int J Biol Sci. 2022 Jan 1;18(1):426-440. doi: 10.7150/ijbs.64640. eCollection 2022. PubMed
Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits. Rodriguez E, Boelaars K, Brown K, Madunic K, van Ee T, Dijk F, Verheij J, Li RJE, Schetters STT, Meijer LL, Le Large TYS, Driehuis E, Clevers H, Bruijns SCM, O'Toole T, van Vliet SJ, Bijlsma MF, Wuhrer M, Kazemier G, Giovannetti E, Garcia-Vallejo JJ, van Kooyk Y. Commun Biol. 2022 Jan 11;5(1):41. doi: 10.1038/s42003-021-02934-0. PubMed
KIAA0319 influences cilia length, cell migration and mechanical cell-substrate interaction. Diaz R, Kronenberg NM, Martinelli A, Liehm P, Riches AC, Gather MC, Paracchini S. Sci Rep. 2022 Jan 14;12(1):722. doi: 10.1038/s41598-021-04539-3. PubMed
S-Nitrosylation of p62 Inhibits Autophagic Flux to Promote alpha-Synuclein Secretion and Spread in Parkinson's Disease and Lewy Body Dementia. Oh CK, Dolatabadi N, Cieplak P, Diaz-Meco MT, Moscat J, Nolan JP, Nakamura T, Lipton SA. J Neurosci. 2022 Apr 6;42(14):3011-3024. doi: 10.1523/JNEUROSCI.1508-21.2022. Epub 2022 Feb 15. PubMed
Melanoma-derived extracellular vesicles mediate lymphatic remodelling and impair tumour immunity in draining lymph nodes. Leary N, Walser S, He Y, Cousin N, Pereira P, Gallo A, Collado-Diaz V, Halin C, Garcia-Silva S, Peinado H, Dieterich LC. J Extracell Vesicles. 2022 Feb;11(2):e12197. doi: 10.1002/jev2.12197. PubMed
PBK/TOPK Is a Favorable Prognostic Biomarker Correlated with Antitumor Immunity in Colon Cancers. Lee DH, Jeong YJ, Won JY, Sim HI, Park Y, Jin HS. Biomedicines. 2022 Jan 27;10(2). pii: biomedicines10020299. doi: 10.3390/biomedicines10020299. PubMed
Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit. Xu M, Zhao Y, Zhang W, Geng M, Liu Q, Gao Q, Shuai L. Cell Prolif. 2022 Apr;55(4):e13209. doi: 10.1111/cpr.13209. Epub 2022 Mar 11. PubMed
Xist spatially amplifies SHARP/SPEN recruitment to balance chromosome-wide silencing and specificity to the X chromosome. Jachowicz JW, Strehle M, Banerjee AK, Blanco MR, Thai J, Guttman M. Nat Struct Mol Biol. 2022 Mar;29(3):239-249. doi: 10.1038/s41594-022-00739-1. Epub 2022 Mar 17. PubMed
ABCA1 Expression Is Upregulated in an EMT in Breast Cancer Cell Lines via MYC-Mediated De-Repression of Its Proximal Ebox Element. Prijic S, Chang JT. Biomedicines. 2022 Mar 2;10(3). pii: biomedicines10030581. doi: 10.3390/biomedicines10030581. PubMed
Structural and genome-wide analyses suggest that transposon-derived protein SETMAR alters transcription and splicing. Chen Q, Bates AM, Hanquier JN, Simpson E, Rusch DB, Podicheti R, Liu Y, Wek RC, Cornett EM, Georgiadis MM. J Biol Chem. 2022 May;298(5):101894. doi: 10.1016/j.jbc.2022.101894. Epub 2022 Apr 1. PubMed
Mesp1 controls the chromatin and enhancer landscapes essential for spatiotemporal patterning of early cardiovascular progenitors. Lin X, Swedlund B, Ton MN, Ghazanfar S, Guibentif C, Paulissen C, Baudelet E, Plaindoux E, Achouri Y, Calonne E, Dubois C, Mansfield W, Zaffran S, Marioni JC, Fuks F, Gottgens B, Lescroart F, Blanpain C. Nat Cell Biol. 2022 Jul;24(7):1114-1128. doi: 10.1038/s41556-022-00947-3. Epub 2022 Jul 11. PubMed
CLP36 promotes p53 deficient sarcoma progression through suppression of atrophin-1 interacting protein-4 (AIP-4)-dependent degradation of YAP1. Lu Y, Mu Y, Chen J, Guan X, Guo L, Wu C. Theranostics. 2022 Jul 4;12(11):5051-5068. doi: 10.7150/thno.72365. eCollection 2022. PubMed
PI3K drives the de novo synthesis of coenzyme A from vitamin B5. Dibble CC, Barritt SA, Perry GE, Lien EC, Geck RC, DuBois-Coyne SE, Bartee D, Zengeya TT, Cohen EB, Yuan M, Hopkins BD, Meier JL, Clohessy JG, Asara JM, Cantley LC, Toker A. Nature. 2022 Aug;608(7921):192-198. doi: 10.1038/s41586-022-04984-8. Epub 2022 Jul 27. PubMed
Effective splicing restoration of a deep-intronic ABCA4 variant in cone photoreceptor precursor cells by CRISPR/SpCas9 approaches. De Angeli P, Reuter P, Hauser S, Schols L, Stingl K, Wissinger B, Kohl S. Mol Ther Nucleic Acids. 2022 Jul 31;29:511-524. doi: 10.1016/j.omtn.2022.07.023. eCollection 2022 Sep 13. PubMed
A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3. Pardal AJ, Bowman AJ. Elife. 2022 Sep 6;11:e81755. doi: 10.7554/eLife.81755. PubMed

Associated Plasmids
Early enforcement of cell identity by a functional component of the terminally differentiated state. Bahrami-Nejad Z, Zhang ZB, Tholen S, Sharma S, Rabiee A, Zhao ML, Kraemer FB, Teruel MN. PLoS Biol. 2022 Dec 5;20(12):e3001900. doi: 10.1371/journal.pbio.3001900. eCollection 2022 Dec. PubMed
mTORC1 regulates a lysosome-dependent adaptive shift in intracellular lipid species. Hosios AM, Wilkinson ME, McNamara MC, Kalafut KC, Torrence ME, Asara JM, Manning BD. Nat Metab. 2022 Dec;4(12):1792-1811. doi: 10.1038/s42255-022-00706-6. Epub 2022 Dec 19. PubMed
Metabolic enzyme LDHA activates Rac1 GTPase as a noncanonical mechanism to promote cancer. Liu J, Zhang C, Zhang T, Chang CY, Wang J, Bazile L, Zhang L, Haffty BG, Hu W, Feng Z. Nat Metab. 2022 Dec;4(12):1830-1846. doi: 10.1038/s42255-022-00708-4. Epub 2022 Dec 19. PubMed
TadA reprogramming to generate potent miniature base editors with high precision. Zhang S, Song L, Yuan B, Zhang C, Cao J, Chen J, Qiu J, Tai Y, Chen J, Qiu Z, Zhao XM, Cheng TL. Nat Commun. 2023 Jan 26;14(1):413. doi: 10.1038/s41467-023-36004-2. PubMed
The ubiquitin ligase TRIM21 regulates mutant p53 accumulation and gain of function in cancer. Liu J, Zhang C, Xu D, Zhang T, Chang CY, Wang J, Liu J, Zhang L, Haffty BG, Zong WX, Hu W, Feng Z. J Clin Invest. 2023 Mar 15;133(6):e164354. doi: 10.1172/JCI164354. PubMed
The UAS thioredoxin-like domain of UBXN7 regulates E3 ubiquitin ligase activity of RNF111/Arkadia. Amhaz S, Boeda B, Chouchene M, Colasse S, Dingli F, Loew D, Henri J, Prunier C, Levy L. BMC Biol. 2023 Apr 7;21(1):73. doi: 10.1186/s12915-023-01576-4. PubMed
Novel genetically engineered mouse models for clear cell renal cell carcinoma. van der Mijn JC, Laursen KB, Fu L, Khani F, Dow LE, Nowak DG, Chen Q, Gross SS, Nanus DM, Gudas LJ. Sci Rep. 2023 May 22;13(1):8246. doi: 10.1038/s41598-023-35106-7. PubMed
CRISPR/Cas9-Mediated Gene Correction in Osteopetrosis Patient-Derived iPSCs. Li D, Ou M, Zhang W, Luo Q, Cai W, Mo C, Liang W, Dai G, Yin L, Zhu P, Tang D, Dai Y. Front Biosci (Landmark Ed). 2023 Jun 30;28(6):131. doi: 10.31083/j.fbl2806131. PubMed
Extracellular vesicle-associated tyrosine kinase-like orphan receptors ROR1 and ROR2 promote breast cancer progression. Irmer B, Efing J, Reitnauer LE, Angenendt A, Heinrichs S, Schubert A, Schulz M, Binder C, Tio J, Hansen U, Geyer C, Gerwing M, Bleckmann A, Menck K. Cell Commun Signal. 2023 Jul 10;21(1):171. doi: 10.1186/s12964-023-01186-1. PubMed
A NRF2 inhibitor selectively sensitizes KEAP1 mutant tumor cells to cisplatin and gefitinib by restoring NRF2-inhibitory function of KEAP1 mutants. Aboulkassim T, Tian X, Liu Q, Qiu D, Hancock M, Wu JH, Batist G. Cell Rep. 2023 Sep 26;42(9):113104. doi: 10.1016/j.celrep.2023.113104. Epub 2023 Sep 12. PubMed
Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice. Li WK, Zhang SQ, Peng WL, Shi YH, Yuan B, Yuan YT, Xue ZY, Wang JC, Han WJ, Chen ZF, Shan SF, Xue BQ, Chen JL, Zhang C, Zhu SJ, Tai YL, Cheng TL, Qiu ZL. Nat Neurosci. 2024 Jan;27(1):116-128. doi: 10.1038/s41593-023-01499-x. Epub 2023 Nov 27. PubMed
DAXX promotes centromeric stability independently of ATRX by preventing the accumulation of R-loop-induced DNA double-stranded breaks. Pinto LM, Pailas A, Bondarchenko M, Sharma AB, Neumann K, Rizzo AJ, Jeanty C, Nicot N, Racca C, Graham MK, Naughton C, Liu Y, Chen CL, Meakin PJ, Gilbert N, Britton S, Meeker AK, Heaphy CM, Larminat F, Van Dyck E. Nucleic Acids Res. 2024 Feb 9;52(3):1136-1155. doi: 10.1093/nar/gkad1141. PubMed
LOXHD1 is indispensable for coupling auditory mechanosensitive channels to the site of force transmission. Wang P, Miller KK, He E, Dhawan SS, Cunningham CL, Grillet N. Res Sq [Preprint]. 2024 Jan 2:rs.3.rs-3752492. doi: 10.21203/rs.3.rs-3752492/v1. PubMed
The growth factor EPIREGULIN promotes basal progenitor cell proliferation in the developing neocortex. Cubillos P, Ditzer N, Kolodziejczyk A, Schwenk G, Hoffmann J, Schutze TM, Derihaci RP, Birdir C, Kollner JE, Petzold A, Sarov M, Martin U, Long KR, Wimberger P, Albert M. EMBO J. 2024 Apr;43(8):1388-1419. doi: 10.1038/s44318-024-00068-7. Epub 2024 Mar 21. PubMed
PALB2-mutated human mammary cells display a broad spectrum of morphological and functional abnormalities induced by increased TGFbeta signaling. Tuppurainen H, Laurila N, Natynki M, Eshraghi L, Tervasmaki A, Erichsen L, Sorensen CS, Pylkas K, Winqvist R, Peltoketo H. Cell Mol Life Sci. 2024 Apr 10;81(1):173. doi: 10.1007/s00018-024-05183-6. PubMed

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