pSpCas9(BB)-2A-Puro (PX459) Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid
48139 with the following enzymes: Age I HF, NotI HF, EcoRV HF, Xbal, EcoRVHf + Xbal
9.2 kb linearized, double digest: 6.4kb and 2.8kb. Looks OK.
