pSpCas9 (PX165) Citations (32)
Originally described in: Genome engineering using the CRISPR-Cas9 system.Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. PubMed Journal
Articles Citing pSpCas9 (PX165)
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| Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed |
| CRISPR/Cas9-mediated GJA8 knockout in rabbits recapitulates human congenital cataracts. Yuan L, Sui T, Chen M, Deng J, Huang Y, Zeng J, Lv Q, Song Y, Li Z, Lai L. Sci Rep. 2016 Feb 25;6:22024. doi: 10.1038/srep22024. PubMed |
| Efficient Generation of Myostatin Gene Mutated Rabbit by CRISPR/Cas9. Lv Q, Yuan L, Deng J, Chen M, Wang Y, Zeng J, Li Z, Lai L. Sci Rep. 2016 Apr 26;6:25029. doi: 10.1038/srep25029. PubMed |
| CRISPR/Cas9-mediated mutation of PHEX in rabbit recapitulates human X-linked hypophosphatemia (XLH). Sui T, Yuan L, Liu H, Chen M, Deng J, Wang Y, Li Z, Lai L. Hum Mol Genet. 2016 Apr 28. pii: ddw125. PubMed |
| CRISPR/Cas9-mediated mutation of tyrosinase (Tyr) 3' UTR induce graying in rabbit. Song Y, Xu Y, Deng J, Chen M, Lu Y, Wang Y, Yao H, Zhou L, Liu Z, Lai L, Li Z. Sci Rep. 2017 May 8;7(1):1569. doi: 10.1038/s41598-017-01727-y. PubMed |
| Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells. Ade CM, Derbes RS, Wagstaff BJ, Linker SB, White TB, Deharo D, Belancio VP, Ivics Z, Roy-Engel AM. Gene. 2018 Feb 5;642:188-198. doi: 10.1016/j.gene.2017.11.033. Epub 2017 Nov 14. PubMed |
| A small cassette enables conditional gene inactivation by CRISPR/Cas9. Guzzardo PM, Rashkova C, Dos Santos RL, Tehrani R, Collin P, Burckstummer T. Sci Rep. 2017 Dec 1;7(1):16770. doi: 10.1038/s41598-017-16931-z. PubMed |
| Multiple homologous genes knockout (KO) by CRISPR/Cas9 system in rabbit. Liu H, Sui T, Liu D, Liu T, Chen M, Deng J, Xu Y, Li Z. Gene. 2018 Mar 20;647:261-267. doi: 10.1016/j.gene.2018.01.044. Epub 2018 Jan 12. PubMed |
| Development of muscular dystrophy in a CRISPR-engineered mutant rabbit model with frame-disrupting ANO5 mutations. Sui T, Xu L, Lau YS, Liu D, Liu T, Gao Y, Lai L, Han R, Li Z. Cell Death Dis. 2018 May 22;9(6):609. doi: 10.1038/s41419-018-0674-y. PubMed |
| A novel rabbit model of Duchenne muscular dystrophy generated by CRISPR/Cas9. Sui T, Lau YS, Liu D, Liu T, Xu L, Gao Y, Lai L, Li Z, Han R. Dis Model Mech. 2018 Jun 4;11(6). pii: dmm.032201. doi: 10.1242/dmm.032201. PubMed |
| Generation and Phenotype Identification of PAX4 Gene Knockout Rabbit by CRISPR/Cas9 System. Xu Y, Wang Y, Song Y, Deng J, Chen M, Ouyang H, Lai L, Li Z. G3 (Bethesda). 2018 Jul 31;8(8):2833-2840. doi: 10.1534/g3.118.300448. PubMed |
| The disrupted balance between hair follicles and sebaceous glands in Hoxc13-ablated rabbits. Deng J, Chen M, Liu Z, Song Y, Sui T, Lai L, Li Z. FASEB J. 2019 Jan;33(1):1226-1234. doi: 10.1096/fj.201800928RR. Epub 2018 Aug 20. PubMed |
| Ex Vivo COL7A1 Correction for Recessive Dystrophic Epidermolysis Bullosa Using CRISPR/Cas9 and Homology-Directed Repair. Izmiryan A, Ganier C, Bovolenta M, Schmitt A, Mavilio F, Hovnanian A. Mol Ther Nucleic Acids. 2018 Sep 7;12:554-567. doi: 10.1016/j.omtn.2018.06.008. Epub 2018 Jun 26. PubMed |
| Dynamic nanoscale morphology of the ER surveyed by STED microscopy. Schroeder LK, Barentine AES, Merta H, Schweighofer S, Zhang Y, Baddeley D, Bewersdorf J, Bahmanyar S. J Cell Biol. 2019 Jan 7;218(1):83-96. doi: 10.1083/jcb.201809107. Epub 2018 Nov 15. PubMed |
| LMNA-mutated Rabbits: A Model of Premature Aging Syndrome with Muscular Dystrophy and Dilated Cardiomyopathy. Sui T, Liu D, Liu T, Deng J, Chen M, Xu Y, Song Y, Ouyang H, Lai L, Li Z. Aging Dis. 2019 Feb 1;10(1):102-115. doi: 10.14336/AD.2018.0209. eCollection 2019 Feb. PubMed |
| Steroid Hormone Function Controls Non-competitive Plasmodium Development in Anopheles. Werling K, Shaw WR, Itoe MA, Westervelt KA, Marcenac P, Paton DG, Peng D, Singh N, Smidler AL, South A, Deik AA, Mancio-Silva L, Demas AR, March S, Calvo E, Bhatia SN, Clish CB, Catteruccia F. Cell. 2019 Apr 4;177(2):315-325.e14. doi: 10.1016/j.cell.2019.02.036. Epub 2019 Mar 28. PubMed |
| Mutations of GADD45G in rabbits cause cleft lip by the disorder of proliferation, apoptosis and epithelial-mesenchymal transition (EMT). Lu Y, Liang M, Zhang Q, Liu Z, Song Y, Lai L, Li Z. Biochim Biophys Acta Mol Basis Dis. 2019 Sep 1;1865(9):2356-2367. doi: 10.1016/j.bbadis.2019.05.015. Epub 2019 May 29. PubMed |
| In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9. Steinecke A, Kurabayashi N, Hayano Y, Ishino Y, Taniguchi H. Cell Rep. 2019 Jul 9;28(2):325-331.e4. doi: 10.1016/j.celrep.2019.06.038. PubMed |
| Proteomic analysis reveals a role for PAX8 in peritoneal colonization of high grade serous ovarian cancer that can be targeted with micelle encapsulated thiostrepton. Hardy LR, Pergande MR, Esparza K, Heath KN, Onyuksel H, Cologna SM, Burdette JE. Oncogene. 2019 Aug;38(32):6003-6016. doi: 10.1038/s41388-019-0842-2. Epub 2019 Jul 11. PubMed |
| CRISPR/Cas9-mediated Disruption of Fibroblast Growth Factor 5 in Rabbits Results in a Systemic Long Hair Phenotype by Prolonging Anagen. Xu Y, Liu H, Pan H, Wang X, Zhang Y, Yao B, Li N, Lai L, Li Z. Genes (Basel). 2020 Mar 11;11(3). pii: genes11030297. doi: 10.3390/genes11030297. PubMed |
Highly Parallel Profiling of Cas9 Variant Specificity.
Schmid-Burgk JL, Gao L, Li D, Gardner Z, Strecker J, Lash B, Zhang F.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
PubMed
Associated Plasmids |
| Cetuximab produced from a goat mammary gland expression system is equally efficacious as innovator cetuximab in animal cancer models. Wang Q, Gavin W, Masiello N, Tran KB, Laible G, Shepherd PR. Biotechnol Rep (Amst). 2020 Sep 22;28:e00533. doi: 10.1016/j.btre.2020.e00533. eCollection 2020 Dec. PubMed |
| CALM supports clathrin-coated vesicle completion upon membrane tension increase. Willy NM, Colombo F, Huber S, Smith AC, Norton EG, Kural C, Cocucci E. Proc Natl Acad Sci U S A. 2021 Jun 22;118(25). pii: 2010438118. doi: 10.1073/pnas.2010438118. PubMed |
Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells.
Ishiguro S, Yachie N.
Methods Mol Biol. 2021;2312:193-223. doi: 10.1007/978-1-0716-1441-9_12.
PubMed
Associated Plasmids |
| Neuromodulatory control of inhibitory network arborization in the developing postnatal neocortex. Steinecke A, Bolton MM, Taniguchi H. Sci Adv. 2022 Mar 11;8(10):eabe7192. doi: 10.1126/sciadv.abe7192. Epub 2022 Mar 9. PubMed |
| Global ubiquitinome profiling identifies NEDD4 as a regulator of Profilin 1 and actin remodelling in neural crest cells. Lohraseb I, McCarthy P, Secker G, Marchant C, Wu J, Ali N, Kumar S, Daly RJ, Harvey NL, Kawabe H, Kleifeld O, Wiszniak S, Schwarz Q. Nat Commun. 2022 Apr 19;13(1):2018. doi: 10.1038/s41467-022-29660-3. PubMed |
| Rational guide RNA engineering for small-molecule control of CRISPR/Cas9 and gene editing. Liu X, Xiong W, Qi Q, Zhang Y, Ji H, Cui S, An J, Sun X, Yin H, Tian T, Zhou X. Nucleic Acids Res. 2022 Apr 21. pii: 6572004. doi: 10.1093/nar/gkac255. PubMed |
| G-quadruplex-guided RNA engineering to modulate CRISPR-based genomic regulation. Liu X, Cui S, Qi Q, Lei H, Zhang Y, Shen W, Fu F, Tian T, Zhou X. Nucleic Acids Res. 2022 Oct 28;50(19):11387-11400. doi: 10.1093/nar/gkac870. PubMed |
| CRISPR/Cas9-mediated deletion of Fam83h induces defective tooth mineralization and hair development in rabbits. Zhang Y, Yang J, Yao H, Zhang Z, Song Y. J Cell Mol Med. 2022 Nov;26(22):5670-5679. doi: 10.1111/jcmm.17597. Epub 2022 Oct 27. PubMed |
| CRISPR-mediated germline mutagenesis for genetic sterilization of Anopheles gambiae males. Smidler AL, Paton DG, Church GM, Esvelt KM, Shaw WR, Catteruccia F. bioRxiv. 2023 Jun 13:2023.06.13.544841. doi: 10.1101/2023.06.13.544841. Preprint. PubMed |
| Foot-and-mouth disease virus structural protein VP3 interacts with HDAC8 and promotes its autophagic degradation to facilitate viral replication. Zhang H, Wang X, Qu M, Li Z, Yin X, Tang L, Liu X, Sun Y. Autophagy. 2023 Jul 14:1-15. doi: 10.1080/15548627.2023.2233847. PubMed |
| The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ. Fuentes LA, Marin Z, Tyson J, Baddeley D, Bewersdorf J. J Cell Biol. 2023 Oct 2;222(10):e202301112. doi: 10.1083/jcb.202301112. Epub 2023 Jul 26. PubMed |
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