pHBintN
(Plasmid
#48135)
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Purpose(Empty Backbone) Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepWH1967K
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Backbone manufacturerSchmiedel et al., 1997
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Growth instructionsFor Bacillus megaterium: Kanamycin and 30 °C (Temperature sensitive origin of replication)
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHBintN was a gift from Dieter Jahn (Addgene plasmid # 48135 ; http://n2t.net/addgene:48135 ; RRID:Addgene_48135) -
For your References section:
Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium. Biedendieck R, Malten M, Barg H, Bunk B, Martens JH, Deery E, Leech H, Warren MJ, Jahn D. Microb Biotechnol. 2010 Jan;3(1):24-37. doi: 10.1111/j.1751-7915.2009.00125.x. Epub 2009 Jun 10. 10.1111/j.1751-7915.2009.00125.x PubMed 21255303
Map uploaded by the depositor.
