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Addgene

pSPAsp-hp
(Plasmid #48122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMM1520
  • Backbone manufacturer
    Malten et al., 2005
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    For Bacillus megaterium: Tetracycline and 37 °C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    predicted optimal signal peptide for Bacillus megaterium protein secretion

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPAsp-hp was a gift from Dieter Jahn (Addgene plasmid # 48122 ; http://n2t.net/addgene:48122 ; RRID:Addgene_48122)

  • For your References section:

    High-yield intra- and extracellular protein production using Bacillus megaterium. Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara E, Jahn D. Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30. 10.1128/AEM.00431-10 PubMed 20435764
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