pAK501
(Plasmid
#48107)
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Purpose(Empty Backbone) lacZ promoter-probe plasmid with pVS1 origin of replication and chloramphenicol resistance
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48107 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepME6010
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Backbone manufacturerDieter Haas
- Backbone size (bp) 8270
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Modifications to backboneInsertion of transcriptional terminator, MCS, lacZ and cat
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAK501 was a gift from Julia Vorholt (Addgene plasmid # 48107 ; http://n2t.net/addgene:48107 ; RRID:Addgene_48107) -
For your References section:
Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria. Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128/AEM.02296-13. Epub 2013 Aug 30. 10.1128/AEM.02296-13 PubMed 23995928
