pQMD
(Plasmid
#48103)
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Purpose(Empty Backbone) Destination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48103 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQM
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Backbone manufacturerAndreas Kaczmarczyk
- Backbone size (bp) 7502
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Modifications to backboneInsertion of Gateway cassette between Xba-Acc65I sites
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Vector typeBacterial Expression
- Promoter PQ5
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Tag
/ Fusion Protein
- myc tag (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Tetracycline, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsGrow in ccdB-resistant E. coli strain
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note that there are some small discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. The depositing lab does not believe these discrepancies affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQMD was a gift from Julia Vorholt (Addgene plasmid # 48103 ; http://n2t.net/addgene:48103 ; RRID:Addgene_48103) -
For your References section:
Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria. Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128/AEM.02296-13. Epub 2013 Aug 30. 10.1128/AEM.02296-13 PubMed 23995928
