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Purpose(Empty Backbone) Cumate-inducible expression of mCherry-tagged fusion protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQF
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Backbone manufacturerAndreas Kaczmarczyk
- Backbone size (bp) 7535
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Modifications to backboneN-terminal 3xFLAG tag replaced by mCherry
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Vector typeBacterial Expression
- Promoter PQ5
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Tags
/ Fusion Proteins
- mCherry (N terminal on backbone)
- 3xFLAG (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQR was a gift from Julia Vorholt (Addgene plasmid # 48099 ; http://n2t.net/addgene:48099 ; RRID:Addgene_48099) -
For your References section:
Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria. Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128/AEM.02296-13. Epub 2013 Aug 30. 10.1128/AEM.02296-13 PubMed 23995928