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Addgene

pQF
(Plasmid #48095)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48095 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCM62
  • Backbone manufacturer
    CJ Marx (Harvard)
  • Backbone size (bp) 6878
  • Modifications to backbone
    Insertion of cymR*, PQ5, MCS with N- and C-terminal 3xFLAG tags and a putative transcriptional terminator (T193*) between PciI-EcoRI sites of pCM62.
  • Vector type
    Bacterial Expression
  • Promoter PQ5
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on backbone)
    • 3xFLAG (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQF was a gift from Julia Vorholt (Addgene plasmid # 48095 ; http://n2t.net/addgene:48095 ; RRID:Addgene_48095)

  • For your References section:

    Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria. Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128/AEM.02296-13. Epub 2013 Aug 30. 10.1128/AEM.02296-13 PubMed 23995928
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