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Addgene

pQF-lacZ
(Plasmid #48094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQF
  • Backbone manufacturer
    Andreas Kaczmarczyk
  • Backbone size w/o insert (bp) 7300
  • Total vector size (bp) 10405
  • Modifications to backbone
    Includes cymR*, PQ5 and lacZ
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E. coli lacZ
  • Promoter PQ5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI, SpeI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ATGGTTGACGGATCGCCG
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQF-lacZ was a gift from Julia Vorholt (Addgene plasmid # 48094 ; http://n2t.net/addgene:48094 ; RRID:Addgene_48094)

  • For your References section:

    Cumate-inducible gene expression system for sphingomonads and other Alphaproteobacteria. Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128/AEM.02296-13. Epub 2013 Aug 30. 10.1128/AEM.02296-13 PubMed 23995928
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