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Addgene

pTXB1-hMeCP2-R270X
(Plasmid #48092)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48092 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTXB1
  • Backbone size w/o insert (bp) 6706
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Methyl-CpG Binding Protein 2 (Rett Syndrome)
  • Alt name
    MeCP2
  • Species
    H. sapiens (human)
  • Mutation
    Mxe intein/chitin binding domain tagged, expresses AT-Hook R270X
  • Entrez Gene
    MECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Ndel (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer T7 Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTXB1-hMeCP2-R270X was a gift from Huda Zoghbi (Addgene plasmid # 48092 ; http://n2t.net/addgene:48092 ; RRID:Addgene_48092)

  • For your References section:

    An AT-hook domain in MeCP2 determines the clinical course of Rett syndrome and related disorders. Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY. Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038. 10.1016/j.cell.2013.01.038 PubMed 23452848
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