pTXB1-hMeCP2
(Plasmid #48091)

• Purpose: Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT
• Depositing Lab: Huda Zoghbi
• Publication: Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038.

Backbone

• Vector backbone: pTXB1
• Backbone size w/o insert (bp): 6706
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Methyl-CpG Binding Protein 2 (Rett Syndrome)
• Alt name: MeCP2
• Species: H. sapiens (human)
• Mutation: Mxe intein/chitin binding domain tagged
• Entrez Gene: MECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ndel (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: T7 Fwd

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry