pCMX-Gal4-hMeCP2-R270X-R111G
(Plasmid
#48086)
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PurposeMammalian expression of Gal4 tagged MeCP2-R270X-R111G
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMX-Gal4
- Backbone size w/o insert (bp) 4500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMethyl-CpG Binding Protein 2 (Rett Syndrome)
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Alt nameMeCP2
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SpeciesH. sapiens (human)
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MutationR111G mutant MeCP2, expresses AT-hook R270X
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Entrez GeneMECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)
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Tag
/ Fusion Protein
- Gal4 (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Gal4 N-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMX-Gal4-hMeCP2-R270X-R111G was a gift from Huda Zoghbi (Addgene plasmid # 48086 ; http://n2t.net/addgene:48086 ; RRID:Addgene_48086) -
For your References section:
An AT-hook domain in MeCP2 determines the clinical course of Rett syndrome and related disorders. Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY. Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038. 10.1016/j.cell.2013.01.038 PubMed 23452848