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PurposeMammalian expression of GFP tagged MeCP2-WT
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA-DEST47
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7780
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMethyl-CpG Binding Protein 2 (Rett Syndrome)
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Alt nameMeCP2
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SpeciesH. sapiens (human)
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Entrez GeneMECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-hMeCP2-GFP was a gift from Huda Zoghbi (Addgene plasmid # 48078 ; http://n2t.net/addgene:48078 ; RRID:Addgene_48078) -
For your References section:
An AT-hook domain in MeCP2 determines the clinical course of Rett syndrome and related disorders. Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY. Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038. 10.1016/j.cell.2013.01.038 PubMed 23452848
