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Addgene

pAGM4673
(Plasmid #48014)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48014 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAGM4301
  • Backbone manufacturer
    self-made
  • Backbone size (bp) 4259
  • Vector type
    Unspecified
  • Selectable markers
    Canthaxanthin operon

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

level 2 cloning vector, replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery to plant cells.

Please note the bacteria should be an orange color when grown on a plate or stab. Since the color needs oxygen to be made, the bacteria may start off as white, but the color will develop (in about one hour) once exposed to sufficient oxygen.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGM4673 was a gift from Sylvestre Marillonnet (Addgene plasmid # 48014 ; http://n2t.net/addgene:48014 ; RRID:Addgene_48014)

  • For your References section:

    A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. 10.1371/journal.pone.0016765 PubMed 21364738
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