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Addgene

pcDNA4/TO-Flag-H3.3
(Plasmid #47980)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47980 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5078
  • Total vector size (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H3F3A
  • Alt name
    H3.3A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    438
  • Mutation
    288 bp T to C synonymous mutation
  • Entrez Gene
    H3-3A (a.k.a. BRYLIB1, H3-3B, H3.3A, H3F3, H3F3A)
  • Promoter CMV promoter with tetracycline operator
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO-Flag-H3.3 was a gift from Bing Zhu (Addgene plasmid # 47980 ; http://n2t.net/addgene:47980 ; RRID:Addgene_47980)

  • For your References section:

    H3.3-H4 tetramer splitting events feature cell-type specific enhancers. Huang C, Zhang Z, Xu M, Li Y, Li Z, Ma Y, Cai T, Zhu B. PLoS Genet. 2013 Jun;9(6):e1003558. doi: 10.1371/journal.pgen.1003558. Epub 2013 Jun 6. 10.1371/journal.pgen.1003558 PubMed 23754967
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