CS2-GFP-Ben
(Plasmid
#47966)
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PurposeExpression of fly Ben
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCS2
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBendless
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SpeciesD. melanogaster (fly)
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Entrez Geneben (a.k.a. Dmel_CG18319, BEN, Ben, Bend, CG18319, Dmel\CG18319, Ubc13, UbcD3, anon-WO03040301.242, nj-262, ubc13)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer atgt ccagcctgcc acgtcgc
- 3′ sequencing primer aaatatgcc gtcgaagact ga (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CS2-GFP-Ben was a gift from Laura Lee (Addgene plasmid # 47966 ; http://n2t.net/addgene:47966 ; RRID:Addgene_47966) -
For your References section:
no poles encodes a predicted E3 ubiquitin ligase required for early embryonic development of Drosophila. Merkle JA, Rickmyre JL, Garg A, Loggins EB, Jodoin JN, Lee E, Wu LP, Lee LA. Development. 2009 Feb;136(3):449-59. doi: 10.1242/dev.027599. 10.1242/dev.027599 PubMed 19141674