pCH001
(Plasmid
#47953)
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PurposetetO-GFP reporter casette
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS306
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Backbone manufacturerATCC
- Total vector size (bp) 4373
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Vector typeYeast Expression ; Tet inducible expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFP
- Promoter T3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
tetO2 inducible expression of GFP.
The selection marker genes have some differences to the published sequence but this does not affect the functioning of the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCH001 was a gift from Attila Becskei (Addgene plasmid # 47953 ; http://n2t.net/addgene:47953 ; RRID:Addgene_47953) -
For your References section:
Stochastic signalling rewires the interaction map of a multiple feedback network during yeast evolution. Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, Becskei A. Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687. 10.1038/ncomms1687 PubMed 22353713
