pR184
(Plasmid #47952)

• Purpose: rtTA positive feedback loop with three tet operators in the GAL1 promoter
• Depositing Lab: Attila Becskei
• Publication: Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.

Backbone

• Vector backbone: pRS306
• Backbone manufacturer: ATCC
• Total vector size (bp): 4373
• Vector type: Yeast Expression ; Tet inducible expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: rtTA
• Promoter: Gal1

Cloning Information

• Cloning method: Unknown
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: Gal1_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TetR is downstream of tetO3W/Gal1 promoter.

The expression in these constructs is driven by tet operators. The GAL1 promoter contains a mutation in the proximal Gal4 BS to have only three Gal4 binding sites. Since the above plasmids (pCH043, pSS63, pPR184) can be used only in delGal4 strains, this does not affect the basal expression in these constructs.