Skip to main content
Addgene

pCH044
(Plasmid #47950)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS306
  • Backbone manufacturer
    ATCC
  • Total vector size (bp) 4373
  • Vector type
    Yeast Expression ; Tet inducible expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    rtTA
  • Promoter Gal2

Cloning Information

  • Cloning method Unknown
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TetR is downstream of Gal2 promoter.

The discrepancies found between the Addgene QC sequence and the full sequence do not affect the functioning of the construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCH044 was a gift from Attila Becskei (Addgene plasmid # 47950 ; http://n2t.net/addgene:47950 ; RRID:Addgene_47950)

  • For your References section:

    Stochastic signalling rewires the interaction map of a multiple feedback network during yeast evolution. Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, Becskei A. Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687. 10.1038/ncomms1687 PubMed 22353713
Related items:
Commonly requested with: