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Addgene

pCMV rat SAPAP1
(Plasmid #47940)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47940 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SAPAP1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Dlgap1 (a.k.a. DAP-1, GKAP/SAPAP1, Gkap, SAPAP1, rGKAP)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SmaI or XmaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV rat SAPAP1 was a gift from Yutaka Hata & Yoshimi Takai (Addgene plasmid # 47940 ; http://n2t.net/addgene:47940 ; RRID:Addgene_47940)

  • For your References section:

    SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. Takeuchi M, Hata Y, Hirao K, Toyoda A, Irie M, Takai Y. J Biol Chem. 1997 May 2;272(18):11943-51. 10.1074/jbc.272.18.11943 PubMed 9115257
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