pClneoMyc human CIN85
(Plasmid #47935)

• Purpose: expresses Myc tagged human CIN85 in mammalian cells
• Depositing Lab: Yutaka Hata
• Publication: Kawata et al J Biochem. 2006 May;139(5):931-9.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIN85
• Species: H. sapiens (human)
• Mutation: contains amino acids 2-665 of human CIN85; D114G
• Entrez Gene: SH3KBP1 (a.k.a. AGMX2, CD2BP3, CIN85, GIG10, HSB-1, HSB1, IMD61, MIG18)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The CIN85 insert in this plasmid contains a D114G mutation when compared to GenBank accession number NP_114098.1. This mutation was not intentional and was present in the original CIN85 clone.