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pClneoMyc human CIN85
(Plasmid #47935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47935 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIN85
  • Species
    H. sapiens (human)
  • Mutation
    contains amino acids 2-665 of human CIN85; D114G
  • Entrez Gene
    SH3KBP1 (a.k.a. AGMX2, CD2BP3, CIN85, GIG10, HSB-1, HSB1, IMD61, MIG18)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The CIN85 insert in this plasmid contains a D114G mutation when compared to GenBank accession number NP_114098.1. This mutation was not intentional and was present in the original CIN85 clone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoMyc human CIN85 was a gift from Yutaka Hata (Addgene plasmid # 47935 ; http://n2t.net/addgene:47935 ; RRID:Addgene_47935)

  • For your References section:

    CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. Kawata A, Iida J, Ikeda M, Sato Y, Mori H, Kansaku A, Sumita K, Fujiwara N, Rokukawa C, Hamano M, Hirabayashi S, Hata Y. J Biochem. 2006 May;139(5):931-9. 10.1093/jb/mvj105 PubMed 16751601
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