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Addgene

pClneoFLAG human Dendrin
(Plasmid #47934)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47934 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo (modified)
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    Oligos encoding FLAG tag were inserted into NheI/EcoRI sites of pClneo vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dendrin-1
  • Species
    H. sapiens (human)
  • Mutation
    contains amino acids 2-711 of human Dendrin
  • Entrez Gene
    DDN
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoFLAG human Dendrin was a gift from Yutaka Hata (Addgene plasmid # 47934 ; http://n2t.net/addgene:47934 ; RRID:Addgene_47934)

  • For your References section:

    CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin. Kawata A, Iida J, Ikeda M, Sato Y, Mori H, Kansaku A, Sumita K, Fujiwara N, Rokukawa C, Hamano M, Hirabayashi S, Hata Y. J Biochem. 2006 May;139(5):931-9. 10.1093/jb/mvj105 PubMed 16751601
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