pT7ddx19gRNA
(Plasmid #47932)

• Purpose: in vitro transcription of ddx19 gRNA
• Depositing Labs: Wenbiao Chen, Susan Wente
• Publication: Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Backbone

• Vector backbone: pT7-gRNA
• Backbone size w/o insert (bp): 2542
• Total vector size (bp): 2526
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ddx19 gRNA
• Species: D. rerio (zebrafish)
• Insert Size (bp): 20
• Entrez Gene: ddx19 (a.k.a. chunp6915, fi21a04, wu:fb24g07, wu:fc59e08, wu:fd14f03, wu:fi21a04)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)
• 5′ sequencing primer: M13 Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/

gRNA target sequence GGCAACAGATTCGTGGGCCC