pSL1180-HR-PUbECFP
(Plasmid #47917)

• Purpose: Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
• Depositing Lab: Leslie Vosshall
• Publication: Vosshall lab Aedes aegypti HR donor plasmid (unpublished)

Backbone

• Vector backbone: pSL1180
• Backbone size w/o insert (bp): 5066
• Total vector size (bp): 5825
• Modifications to backbone: Includes Aedes aegypti polyubiquitin (PUb) promoter and SV40 polyadenylation sequence.
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ECFP
• Alt name: Enhanced Cyan Fluorescent Protein
• Species: Aequorea victoria
• Insert Size (bp): 759
• Promoter: Aedes aegypti polyubiquitin (PUb)
• Tag / Fusion Protein:
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13_reverse_primer
• 3′ sequencing primer: M13_forward20_primer

Resource Information

• Supplemental Documents:
  • pSL1180-HR-PUbECFP Genbank file
  • pSL1180-HR-PUbECFP_Map
• A portion of this plasmid was derived from a plasmid made by: Aedes aegypti PUb promoter used in pSL1180-HR-PUbECFP was PCR-amplified from pMos-3xP3DsRed-PUb-EGFP-attP (a kind gift of Dr Zach Adelman, Virginia Tech). Anderson et al. (2010) Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti. Insect Mol Biol. 19:441-9.
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by Dr Conor McMeniman, Vosshall lab.