SP6-sgRNA-scaffold
(Plasmid #47912)

• Purpose: (Empty Backbone) Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
• Depositing Lab: Paul Sternberg
• Publication: Chiu et al Genetics. 2013 Aug 26.

Backbone

• Vector backbone: Bluescript
• Backbone manufacturer: Stratagene
• Backbone size (bp): 3000
• Vector type: Worm Expression, CRISPR
• Promoter: SP6
• Tag / Fusion Protein:
  • sgRNA 3' end (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Contains sequence adapted from Addgene plasmid #41824 "gRNA Empty Vector"
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry